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How to culture daphnia?
November 8, 2014
7:11 pm
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F. Lange Santos
Mococa, northeast of the state of São Paulo, Brazil.
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Hello guys,

I have a growing water fleas a few weeks ago.

I'm having some doubts because my culture is infested with ostracods, and want a culture without these animals.

They hinder the multiplication of daphnia?

What best food that can be given to them? Spirulina or dry yeast?

How you take care of her?

Felipe Lange

November 10, 2014
12:26 am
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BallAquatics
Tremont City Ohio, USA pop.640
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I feed mine a combination of dry pea flour, spirulina algae, cholera algae, active dry yeast, a pinch of paprika and green water.  I feed twice and on the third day harvest and do a large water change at the same time.  With four cultures running I get a large daily harvest.

Daphnia harvest video.....

Dennis

November 12, 2014
2:31 am
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F. Lange Santos
Mococa, northeast of the state of São Paulo, Brazil.
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It's very interesting the way they cultivate Daphnia.

Make partial water changes while making them harvest is great.

Thank you for your attention.

Felipe Lange

November 14, 2014
12:56 pm
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coelacanth
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It'll be very hard to eliminate ostracods from a culture so you'd need to start again, and containers would need to be thoroughly cleaned as some produce egg capsules that are highly resiatnt to dessication. However, I seem to remember from papers on killifish diet that ostracods can feature significantly, so they might not need to go to waste. Some characins also easily manage them, Astyanax seem to think of them as moving pistachios...
It's true though that many fish are pretty ribbush at eating them.

November 14, 2014
9:43 pm
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BillT
Eugene, Oregon
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I assume that ostracods are present in my Moina cultures (basically a smaller version of Daphnia). They certainly are in the fish tanks. However, my cultures do fine.

I figure the ostracods are mostly at the bottom whereas the Moina and all over the place which should reduce any competition. But I also siphon debris off the bottom 2 or times a week. This should remove a good proportion of any ostracods that might be present.

Bill Trevarrow [email protected]
November 15, 2014
12:22 am
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BallAquatics
Tremont City Ohio, USA pop.640
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Fish filmed spitting ostracod 'fireworks'.....

 

http://www.bbc.co.uk/nature/28838200

 

Dennis

November 15, 2014
2:29 am
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BillT
Eugene, Oregon
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That's pretty cool Dennis.

I have only seem ostracods at the bottom of my tanks and they seem to be much smaller than the ones in the movie.

Bill Trevarrow [email protected]
November 15, 2014
4:11 pm
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F. Lange Santos
Mococa, northeast of the state of São Paulo, Brazil.
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Guys, unfortunately my daphnia culture collapsed, do not know why yet ...

Only left here even a culture of earthworms and peanut beetles (Palembus dermestoides).

Here in the region is ridiculous, so difficult to buy starts of water fleas.

Felipe Lange

November 16, 2014
1:24 pm
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BallAquatics
Tremont City Ohio, USA pop.640
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I would change most of the water or take the mulm from the bottom of the tank and put it in a new container.  If the conditions are good, the ephippia should hatch within 4 to 8 days.  Of course, if you accidentally poisoned the culture they may not be able to make a come back.  Daphnia are very resilient, and can often rise from the dead like the mythical Phoenix.

Best of luck getting your culture back.

Dennis

November 17, 2014
6:14 pm
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coelacanth
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Indeed, or find an ephemeral pool somewhere, maybe near a livestock paddock. Alternatively, talk to the nearest University that does biological sciences and aks if they have any.

November 18, 2014
3:48 am
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deepinpeat
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F. Lange Santos said
Guys, unfortunately my daphnia culture collapsed, do not know why yet ...

Daphnia cultures collapse quite easily usually when you overfeed. When starting the culture you need to feed very little quite often. If you feel like you feed very little use tests to keep a track of how quickly the nitrogen substances concentrations go up and what is to much. It is very good to have live algae cultures to feed them becouse it is way safer than any "dead" food that decomposes quickly. Use as big tanks as possible and try big bubble aeration. High oxygen levels will allow for higher densities and easier feeding aftewords + the food is equally suspended. Using waste water from tanks, that contains some nitrification bacteria helps too. Always have couple cultures running and keep one low density culture for safety. Moina is much easier to culture and probably better if you do not need big mouthfuls for specific reasons. Still not as easy as people think without experiance.

November 18, 2014
4:38 pm
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plesner
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I've been running a few daphnia cultures continuously since February 2011. I feed them nothing but green water, which I also culture. They're kept in two 12 litre and three 25 litre tanks at 20-24°C. When the number of daphnia in a culture begins to drop noticably, I siphon out most of the debris from the bottom and in the process remove about half of the water, which I then top up with fresh water - this happens once every 4-6 months. The water and debris, which I have siphoned off into a bucket, is left for a few days. I can usually harvest a decent amount of daphnia from that bucket, too.

I culture scuds (Hyalella azteca) in neighbouring tanks and all my daphnia cultures contain some scuds as well, but most of my fish eat them anyway.

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