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Effects of Body-Color Mutations on Vitality: An Attempt to Establish Easy-to-Breed See-Through Medaka Strains by Outcrossing
September 13, 2013
2:48 am
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BillT
Eugene, Oregon
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September 10, 2010
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in G3: Genes, Genomes, Genetics

http://www.g3journal.org/conte.....l.pdf+html

 

ABSTRACT “See-through” strains of medaka are unique tools for experiments: their skin is transparent,
and their internal organs can be externally monitored throughout life. However, see-through fish are less
vital than normally pigmented wild-type fish, which allows only skilled researchers to make the most of their
advantages. Expecting that hybrid vigor (heterosis) would increase the vitality, we outcrossed two seethrough
strains (SK2 and STIII) with a genetically distant wild-type strain (HNI). Fish with the see-through
phenotypes were successfully restored in the F2 generation and maintained as closed colonies. We verified
that genomes of these hybrid see-through strains actually consisted of approximately 50% HNI and approximately
50% SK2 or STIII alleles, but we could not obtain evidence supporting improved survival of
larvae or fecundity of adults, at least under our breeding conditions. We also found that four of the five seethrough
mutations (bg8, i-3, gu, and il-1 but not lf ) additively decrease viability. Given that heterosis could
not overwhelm the viability-reducing effects of the see-through mutations, easy-to-breed see-through
strains will only be established by other methods such as conditional gene targeting or screening of new
body-color mutations that do not reduce viability.

 

 

Bill Trevarrow [email protected]
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