LOGIN

RSS Facebook Twitter YouTube
GLOSSARY       

SEARCHGLOSSARY

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

PROFILESEARCH

Avatar

Please consider registering
guest

sp_LogInOut Log In sp_Registration Register

Register | Lost password?
Advanced Search

— Forum Scope —




— Match —





— Forum Options —





 

Minimum search word length is 4 characters - maximum search word length is 84 characters

sp_Feed Topic RSS sp_TopicIcon
Examination of a Culturable Microbial Population from the Gastrointestinal Tract of the Wood-Eating Loricariid Catfish Panaque nigrolineatus
September 25, 2013
7:55 pm
Avatar
Matt
Málaga, Spain
Admin
Forum Posts: 8239
Member Since:
June 13, 2011
sp_UserOfflineSmall Offline

Open access in Diversity

Abstract

Fish play a critical role in nutrient cycling and organic matter flow in aquatic environments. However, little is known about the microbial diversity within the gastrointestinal tracts that may be essential in these degradation activities. Panaque nigrolineatus is a loricariid catfish found in the Neotropics that have a rare dietary strategy of consuming large amounts of woody material in its natural environment. As a consequence, the gastrointestinal (GI) tract of P. nigrolineatus is continually exposed to high levels of cellulose and other recalcitrant wood compounds and is, therefore, an attractive, uncharacterized system to study microbial community diversity. Our previous 16S rRNA gene surveys demonstrated that the GI tract microbial community includes phylotypes having the capacity to degrade cellulose and fix molecular nitrogen. In the present study we verify the presence of a resident microbial community by fluorescence microscopy and focus on the cellulose-degrading members by culture-based and 13C-labeled cellulose DNA stable-isotope probing (SIP) approaches. Analysis of GI tract communities generated from anaerobic microcrystalline cellulose enrichment cultures by 16S rRNA gene analysis revealed phylotypes sharing high sequence similarity to known cellulolytic bacteria including ClostridiumCellulomonasBacteroides, Eubacterium and Aeromonas spp. Related bacteria were identified in the SIP community, which also included nitrogen-fixing Azospirillum spp. Our ability to enrich for specialized cellulose-degrading communities suggests that the P. nigrolineatus GI tract provides a favorable environment for this activity and these communities may be involved in providing assimilable carbon under challenging dietary conditions.

Cake or death?
September 27, 2013
12:37 am
Avatar
BillT
Eugene, Oregon
Veteran
Forum Posts: 229
Member Since:
September 10, 2010
sp_UserOfflineSmall Offline

Wow. Termites of the river?

Bill Trevarrow [email protected]
September 27, 2013
6:27 pm
Avatar
Gloups
New Member
Forum Posts: 7
Member Since:
December 5, 2012
sp_UserOfflineSmall Offline

BillT said
Wow. Termites of the river?

Yes wow a nitrogen-fixing fish, just like some termites.

Forum Timezone: Europe/Paris

Most Users Ever Online: 246

Currently Online:
1 Guest(s)

Currently Browsing this Page:
1 Guest(s)

Devices in use: Desktop (1)

Top Posters:

Stefan: 1567

Plaamoo: 1256

mikev: 1134

Malti: 1099

Mark Duffill: 1012

Member Stats:

Guest Posters: 0

Members: 30493

Moderators: 0

Admins: 2

Forum Stats:

Groups: 4

Forums: 10

Topics: 4603

Posts: 36639

Newest Members: Qwooks, Terry Lambert 50, Monjurul Hasan, Chuno1, reptilesforever

Administrators: dunc: 1323, Matt: 8239