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Culturing Daphnia
February 20, 2012
11:39 am
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Matt
Málaga, Spain
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Does anyone have experience with ZM's Spirulina supplement for feeding live Daphnia? Failing that, any suggestions for proprietary products?

This is for the SEI project and we have several pools, each with a diameter of 3 metres for live food production this year.

Cake or death?
February 20, 2012
5:16 pm
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Bully
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TA Aquaculture's spirulina product is cheaper. I've not used it for Daphnia but do use it for marine copepods.

What's the volume of the pools? You might do better growing live algae in the ponds first, that would be the best choice, in my opinion.

February 20, 2012
5:26 pm
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Colin
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I use spirulina for Xenopus tadpoles and Artemia - have been using the New Era stuff and getting good results.

It's very scientific - when the water isn't green anymore I add more spirulina /smile.gif" style="vertical-align:middle" emoid=":)" border="0" alt="smile.gif" />

bit of trial and error in beginning

February 20, 2012
10:02 pm
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ste12000
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Hi Matt, i should imagine that the costs would soon mount up using commercial spirulina for feeding daphnia..

I have excellent results in the spring/summer using small amounts of manures.. Horse manure in a mesh bag would do the trick, i also use chicken manure pellets designed for Garden use, clean, easy to use and last a long time..

I run two 200litre pools of daphnia and by rotation in summer i can culture enough food to feed 75 tanks several times per week.

February 21, 2012
12:34 am
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Graham Ramsay
Blairgowrie - UK
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I used sheep droppings. Kept the last culture going for 3 years before the hard frost last winter shattered the old tank I was using. Just make sure it gets plenty of indirect sunlight.

February 21, 2012
8:53 am
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Matt
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Thanks lads, and that's very interesting about the use of, well, poo. Are we talking fresh or dried?

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February 21, 2012
9:02 am
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Colin
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rabbit droppings should be fine too then? That used to be used as an aquarium plant fertiliser by just pushing the pellets down into the substrate next to the roots

February 21, 2012
12:19 pm
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ste12000
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QUOTE (Colin @ Feb 21 2012, 08:45 AM) < {POST_SNAPBACK}>
rabbit droppings should be fine too then? That used to be used as an aquarium plant fertiliser by just pushing the pellets down into the substrate next to the roots

/thumbs_up.gif" style="vertical-align:middle" emoid=":thumbsup:" border="0" alt="thumbs_up.gif" /> /thumbs_up.gif" style="vertical-align:middle" emoid=":thumbsup:" border="0" alt="thumbs_up.gif" /> /thumbs_up.gif" style="vertical-align:middle" emoid=":thumbsup:" border="0" alt="thumbs_up.gif" />

Brings back memories.. I used to do this. The one benefit of a sister owning a rabbit..

Matt, im just scanning the relevant pages from the Bible that is the TFH Encyclopedia of live foods..Send me your email address and ill send it across..

February 21, 2012
3:54 pm
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Colin
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LOL

is that the Hellweg book?

February 21, 2012
4:16 pm
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Matt
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There are horses grazing part of the delta and we actually had to install an electric fence around the last natural habitat of Aphanius to keep them from cr*pping in the water so we should be able to do this I think...

Ste, got it, thanks a lot!

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February 21, 2012
6:43 pm
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ste12000
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QUOTE (Colin @ Feb 21 2012, 03:37 PM) < {POST_SNAPBACK}>
LOL

is that the Hellweg book?

No mate, i never bothered buying that one.. I use the O'masters book and its a excellent source of info..

DSCF7198-1.jpgImage Enlarger

Matt, no problem, i hope its helpful to the cause...

Heres my small setup, theres another 200litre tank behind the poly boxes..It will be tidied up before spring /blush.gif" style="vertical-align:middle" emoid=":blush:" border="0" alt="blush.gif" />
DSCF7179.jpgImage Enlarger

These are the Chicken manure pellets i use.. They dont smell too clever but are great for the job, clean, easy to use and fairly cheap..
DSCF7181.jpgImage Enlarger


DSCF7182.jpgImage Enlarger

February 21, 2012
7:44 pm
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Graham Ramsay
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Do you not cover the tank Ste? I had to as water beetles would unerringly find a great place to set up home.

February 21, 2012
8:07 pm
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ste12000
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QUOTE (mummymonkey @ Feb 21 2012, 07:27 PM) < {POST_SNAPBACK}>
Do you not cover the tank Ste? I had to as water beetles would unerringly find a great place to set up home.

Hi Graham, no mate ive never covered it, ive never had any water beatles(Touch wood) just a healthy population of Daphnia and plenty of mosquito larvae in the summer months.

March 1, 2012
7:09 am
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Nikhil
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Matt at my farm we culture Daphnia in 10'X10'X3' ponds which are lined with HDPE plastic sheets. We add about 20Kg of fresh chicken manure to this and fill in water. The water will turn green in 2-4 days and then you can add your starter culture. When the plankton levels/Green water gets transparent i.e. visibility of more than 30cm we add about 26Ltrs note not Kgs of Chicken manure. We get enough from 4 such pits to feed about 20000 livebearers of different sizes twice a day and good amounts. You should also get Bloodworms in this if you keep the water level lower about 8inch.

If the water gets too green and gets a crystal like surface add a few floating plants which will suck the excess right out and then can be used as shelter for your livebearers.

March 1, 2012
1:25 pm
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Matt
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Cheers Nik, looks like some form of poo is the way forward! /laugh.gif" style="vertical-align:middle" emoid="Laugh" border="0" alt="laugh.gif" />

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March 2, 2012
12:17 am
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BallAquatics
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QUOTE (Matt @ Feb 20 2012, 06:22 AM) < {POST_SNAPBACK}>
Does anyone have experience with ZM's Spirulina supplement for feeding live Daphnia? Failing that, any suggestions for proprietary products?


The University of Florida offers up these choices for feeding.....

The following quantity of fertilizer materials should be added initially for each 100 gallons
(379 L) of water. Additional feed or fertilizer, approximately 50-100% of the initial amount, should be added about 5 days later.

Yeast: 0.3-0.5 ounces (8.5-14.2 g) of baker's yeast.
Yeast and mineral fertilizer: 0.3-0.5 ounces (8.5-14.2 g) of yeast, and 0.5 ounces (14.2 g) of ammonium nitrate.
Alfalfa, bran and yeast: 1.5 ounces (42.5 g) of alfalfa pellets or meal, 1.5 ounces (42.5 g) of wheat or rice bran, and 0.3 ounces (8.5 g) of yeast.
Cow manure or sewage sludge, bran and yeast: 5 ounces (142 g) of dried manure or sewage sludge, 1.5 ounces (42.5 g) of wheat or rice bran, and 0.3 ounces (8.5 g) of yeast.
Cow manure or sewage sludge, cotton seed meal and yeast: Use 5 ounces (142 g) of dried manure or sewage sludge, 1.5 ounces (42.5 g) of cotton seed meal and 0.3 ounces (8.5 g) of yeast.
Horse or cow manure or sewage sludge: Combine 20 ounces (567 g) of dried manure or sewage sludge.
Chicken or hog manure: Combine 6 ounces (170 g) of dried manure.
Yeast and spirulina powder: 0.2 ounces (6 g) bakers yeast , 0.1 ounces (3 g) spirulina powder. Add this amount for the first two days, and then every other day until culture is harvested. Note: Add warm water to yeast and spirulina powder and let sit for 30 minutes. Stir and pour contents through a brine shrimp net into the Moina culture. The net will catch un-dissolved yeast and extend the life of the culture.

The entire document is available here... http://dallasdiscus.com/daphni.....rticle.htm

Dennis

March 2, 2012
8:06 am
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Matt
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Thanks Dennis, duly downloaded. /smile.gif" style="vertical-align:middle" emoid=":)" border="0" alt="smile.gif" />

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March 25, 2012
11:44 am
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ste12000
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Just a note to this interesting thread, since reading the above paper on feeding daphnia cultures ive been feeding mine outside with a disolved yeast solution, just enough to slightly cloud the water.

Its clearing again within two days so im feeding 3-4 times per week... The tubs are heaving with newly born and adult daphnia. Im purposely not harvesting any just yet but its really thriving, the current warm spring weather and the new feeding regime is definately producing great results.

March 25, 2012
3:50 pm
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BallAquatics
Tremont City Ohio, USA pop.640
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QUOTE (ste12000 @ Mar 25 2012, 07:27 AM) < {POST_SNAPBACK}>
The tubs are heaving with newly born and adult daphnia. Im purposely not harvesting any just yet but its really thriving, the current warm spring weather and the new feeding regime is definately producing great results.


That's great news, thanks for the update ste12000. Be careful to not let the population get too great... in the past I have been caught admired my handy work only to have a culture crash from not harvesting enough of the results.

Dennis

March 25, 2012
5:30 pm
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ste12000
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QUOTE (BallAquatics @ Mar 25 2012, 04:33 PM) < {POST_SNAPBACK}>
That's great news, thanks for the update ste12000. Be careful to not let the population get too great... in the past I have been caught admired my handy work only to have a culture crash from not harvesting enough of the results.

Dennis

Wise words Dennis, cheers..

Upon closer inspection i also have a healthy culture of cyclops in one of the containers.. Bonus.

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