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Effects of Body-Color Mutations on Vitality: An Attempt to Establish Easy-to-Breed See-Through Medaka Strains by Outcrossing

Home Forums Ichthyology Effects of Body-Color Mutations on Vitality: An Attempt to Establish Easy-to-Breed See-Through Medaka Strains by Outcrossing

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    BillT
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    in G3: Genes, Genomes, Genetics

    http://www.g3journal.org/content/3/9/1577.full.pdf+html

     

    ABSTRACT “See-through” strains of medaka are unique tools for experiments: their skin is transparent,
    and their internal organs can be externally monitored throughout life. However, see-through fish are less
    vital than normally pigmented wild-type fish, which allows only skilled researchers to make the most of their
    advantages. Expecting that hybrid vigor (heterosis) would increase the vitality, we outcrossed two seethrough
    strains (SK2 and STIII) with a genetically distant wild-type strain (HNI). Fish with the see-through
    phenotypes were successfully restored in the F2 generation and maintained as closed colonies. We verified
    that genomes of these hybrid see-through strains actually consisted of approximately 50% HNI and approximately
    50% SK2 or STIII alleles, but we could not obtain evidence supporting improved survival of
    larvae or fecundity of adults, at least under our breeding conditions. We also found that four of the five seethrough
    mutations (bg8, i-3, gu, and il-1 but not lf ) additively decrease viability. Given that heterosis could
    not overwhelm the viability-reducing effects of the see-through mutations, easy-to-breed see-through
    strains will only be established by other methods such as conditional gene targeting or screening of new
    body-color mutations that do not reduce viability.

     

     

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